CD200R Effector Reporter cell
CBP74179
詢(xún) 價(jià)
索取Protocol
產(chǎn)品描述
產(chǎn)品數據庫
| I. Background | |
| CD200R作為CD200的同源配體，也是一種IgSF蛋白，在小鼠和人類(lèi)中，CD200R的表達模式相似，均在巨噬細胞、中性粒細胞和肥大細胞中高表達。與大多數IgSF受體不同，CD200R缺乏ITIM結構域，然而，它的67個(gè)氨基酸組成的細胞質(zhì)尾部含有三個(gè)酪氨酸殘基，第三個(gè)酪氨酸殘留位于NPXY基序內，該基序在CD200R結合配體后會(huì )被磷酸化，這導致其并不直接招募SHP1,SHP2等蛋白質(zhì)酪氨酸磷酸酶或磷酸化后的肌醇磷酸酶（SHIP），而是招募酪氨酸激酶下游銜接蛋白Dok-2和1，然后再與RasGAP和SHIP結合。 | |
| II. Introduction | |
| Expressed gene: | CD200R |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin+10ug/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
|
Figure 1. Recombinant CD200R Effector Reporter cell constitutively expressing CD200R. |
|
|
Figure 2. Dose Response of CD200R Blocking Ab in CD200R Effector Reporter Cells (C34) With CD200 Target cells (C18). |
|
